Le dogme fondamental de la transcription (ADN => ARN) s'effondre en mars/mai 2011



Le dogme fondamental qui affirmait que l'ARNm (mRNA) est une copie conforme de l'ADN n'est plus exact depuis mai 2011... Une expérience menée sur 27 personnes l'a démontré en mars 2011, puis a été accepté pour publication le 10 mai 2011...


Cela signifie que l'ARN produit ne respecte pas l'ADN, et que d'autres mécanisme [inconnus] procèdent pour la formation de l'ARN, qui doit donner ensuite les protéines et enzymes (François Jacob, 1966), à la base absolue du fonctionnement général de la cellule.



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source :
ScienceMag :
http://www.sciencemag.org/content/333/6038/53




Article d'origine en anglais
 TRADUCTION EN FRANCAIS
Published Online May 19 2011
Science 1 July 2011:
Vol. 333 no. 6038 pp. 53-58
DOI: 10.1126/science.1207018
  • Research Article

Widespread RNA and DNA Sequence Differences in the Human Transcriptome

 Publié ne ligne le 19 mai 2011
SCIENCE, 1er juillet 2011
Volume 333 N°6038
DOI 10 11 26 / Science 1 207 018

Différenciation généralisée des séquences d'ADN-ARN dans le transcriptome humain

[TRANSCRIPTÔME / définition :
= structures dynamiques du phénomène de la
transcription ADN => ARN chez l'Homme]


  1. Mingyao Li1,*,
  2. Isabel X. Wang2,*,
  3. Yun Li3,4,
  4. Alan Bruzel2,
  5. Allison L. Richards5,
  6. Jonathan M. Toung6,
  7. Vivian G. Cheung2,7,8,†
+ Author Affiliations

  1. 1Departments of Biostatistics and Epidemiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
  2. 2Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA.
  3. 3Department of Genetics, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
  4. 4Department of Biostatistics, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
  5. 5Cell and Molecular Biology Graduate Program, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
  6. 6Genomics and Computational Biology Graduate Program, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
  7. 7Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
  8. 8Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
  1. ↵†To whom correspondence should be addressed. E-mail: vcheung@mail.med.upenn.edu
  1. ↵* These authors contributed equally to this work.


  1. Mingyao Li1,*,
  2. Isabel X. Wang2,*,
  3. Yun Li3,4,
  4. Alan Bruzel2,
  5. Allison L. Richards5,
  6. Jonathan M. Toung6,
  7. Vivian G. Cheung2,7,8,†
7,8,† + Author Affiliations

  1. 1Departments of Biostatistics and Epidemiology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
  2. 2Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA.
  3. 3Department of Genetics, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
  4. 4Department of Biostatistics, University of North Carolina School of Medicine, Chapel Hill, NC 27599, USA.
  5. 5Cell and Molecular Biology Graduate Program, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
  6. 6Genomics and Computational Biology Graduate Program, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
  7. 7Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
  8. 8Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
  1. ↵†To whom correspondence should be addressed. E-mail: vcheung@mail.med.upenn.edu
  1. ↵* These authors contributed equally to this work.

Abstract

The transmission of information from DNA to RNA is a critical process. We compared RNA sequences from human B cells of 27 individuals to the corresponding DNA sequences from the same individuals and uncovered more than 10,000 exonic sites where the RNA sequences do not match that of the DNA. All 12 possible categories of discordances were observed. These differences were nonrandom as many sites were found in multiple individuals and in different cell types, including primary skin cells and brain tissues. Using mass spectrometry, we detected peptides that are translated from the discordant RNA sequences and thus do not correspond exactly to the DNA sequences. These widespread RNA-DNA differences in the human transcriptome provide a yet unexplored aspect of genome variation.

  • Received for publication 3 March 2011.
  • Accepted for publication 10 May 2011.

Résumé

La transmission d'informations à partir d'ADN à l'ARN est un processus critique. Comparé séquences d'ARN à partir de nous, les cellules B humaines de 27 individuos à des séquences d'ADN correspondantes auprès des mêmes personnes et découvert plus de 10.000 sites de exoniques séquences d'ARN Lorsque le ne correspond pas à celle de l'ADN. Tous les 12 catégories possibles de discordances ont été observées. Ces non aléatoire autant de différences Were Were trouvé dans plusieurs sites et des personnes dans différents types cellulaires, y compris les cellules de la peau et les tissus cérébraux primaires. Utilisation de la spectrométrie de masse, nous avons détecté peptides sont traduits à partir du That discordants des séquences d'ARN ne correspondent pas à Malthus et à des séquences d'ADN Exactement. Ces ARN-ADN à grande échelle chez l'homme diferencias transcriptome Fournir un aspect encore inexploré de la variation du génome.
  • Reçu pour publication le 3 mars 2011
  • Accepté pour publication le 10 mai 2011

Responses to this article

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Read the Full Text

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Les réponses à cet article
  • Etude en génétique sur la discordance graphique dans les susceptibilités aux maladies
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    • Lorraine Chalifour, Morris Schweitzer